Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG.It also reacts with the light chains of other mouse immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) Suggested Working Concentration or Dilution Range:

1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substratesDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Horseradish Peroxidase

Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Images & References

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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Dharmacon公司创立于1995年，是世界领先的siRNA产品供应商和RNA干扰技术服务商，是siRNA诱导的基因沉默功能研究和为人类疾病基因治疗提供特异性高品质试剂的最重要资源。

Dharmacon以其强大的科研实力，为广大客户提供一系列优秀产品和尖端技术，SMARTselectionTM技术、SMARTpool®技术、ON-TARGETplusTM技术和siGENOMETM siRNA产品即是其典型代表。这些技术和产品为研究基因的功能及其在生命活动中的作用提供了强大的工具。

目前Dharmacon产品是RNA干扰领域里应用最为广泛的一类产品。最新统计显示，全球顶尖杂志Nature系列期刊文章中使用Dharmacon®产品的数目突破1000篇，这一数据遥遥领先于RNAi领域内的其它品牌。

为了更好的服务于全球科学家，Dharmacon专家团队创立了“RNA干扰全球倡议组织”，其由20多个分布于北美、欧洲和亚洲的著名非盈利性生物化学研究机构组成。该组织的成立旨在随着全基因组siRNA文库在全球范围内日益广泛的使用，使科学家们对疾病机制有更详细的了解，以提高新药研发的效率，加快生物医学研究的步伐。

三大技术：

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③ON-TARGETplusTM--显著降低基因沉默中的脱靶效应，效率高达90%以上。

 

六类产品：

①AccellTMsiRNA --该产品是RNAi研究领域具革命性的一项创举，它无需任何传统介质 （如转染试剂、病毒载体和电转）即可将siRNA高效递送进入任何类型细胞；

②MiRIDIAN®microRNA Mimics & hairpin Inhibitors --设计先进，调节功能异常强大，实验结果高度可重复。

③siGENOMETMsiRNA--四大组合，打造了全球最全面、最可靠的siRNA基因沉默平台；

④DharmaFECTTM转染试剂--五种选择，可满足siRNA对众多细胞的高效超低毒转染；

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